Q&A: How to distinguish plasma membrane proteins from others in Red blood Cells?
Question by funnyfriend: How to distinguish plasma membrane proteins from others in Red blood Cells?
Hi! I am writing a lab report and I am having trouble with one part. During the lab we used SDS-PAGE (Gel electro) to present plasma membrane proteins. You can not tell the proteins apart for certain using this. I was wondering it there was a method of some sort that you can use to tell exactly what proteins it was that we found in the red blood cells. If any one know!!! Please Help
Best answer:
Answer by Ishan26
Presence of characteristic antigens on the plasma membrane of RBC will help distinguish it from those of inerior membranes.
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You can do SDS-PAGE followed by a Western blot (immunoblot). You run the protein out on the gel, then transfer them to a membrane and probe the membrane for different proteins with antibodies that are specific for them.
You could also do SDS-PAGE, stain the gel with Coumassie Blue or some other stain, cut out the bands or band you’re interested in and send it for mass spec analysis. From this, you could get a protein sequence and using a protein database, figure out which protein it is.
Finally, you could do Fluorescence-Activated Cell Sorting if you had antibodies for the proteins you’re interested in.